期刊
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
卷 394, 期 1, 页码 95-105出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-009-2692-1
关键词
Cell systems/single cell analysis; Electrochemical sensors; Bioanalytical methods; Reactive oxygen species; Reactive nitrogen species; Cell signaling
Reactive oxygen species (ROS) and reactive nitrogen species (RNS) play a crucial role in chemical signaling processes of biological cells. Electrochemistry is one of the rare methods able to directly detect these species. ROS and RNS can be monitored in the local microenvironment of cells in real time at the site where the actual signaling takes place. This review presents recent advances made with amperometric electrochemical techniques. Existing challenges for the quantification of ROS and RNS in biological systems are discussed to promote the development of innovative and reliable cell-based assays.
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