期刊
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
卷 394, 期 7, 页码 1803-1809出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-009-2883-9
关键词
Surface-enhanced Raman spectroscopy; Living cell analysis; Raman spectroscopic imaging; Yeast cell wall; Spectroscopy/Theory; Laser spectroscopy; Cell systems/Single cell analysis
The surface of a living yeast cell (Saccharomyces cerevisiae strain W303-1A) has been labeled with silver (Ag) nanoparticles that can form nanoaggregates which have been shown to have surface-enhanced Raman scattering (SERS) activity. The cell wall of a single living yeast cell has been imaged by use of a Raman microspectroscope. The SERS spectra measured from different Ag nanoaggregates were found to be different. This can be explained on the basis of detailed spectral interpretation. The SERS spectral response originates from mannoproteins which cover the outermost regions of the yeast cell wall. Analysis of SERS spectra from the cell wall and the extracted mannoproteins from the yeast has been performed for the clarification of variation in SERS spectra.
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