期刊
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
卷 395, 期 5, 页码 1325-1334出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-009-2822-9
关键词
T-2 toxin; HT-2 toxin; Carboxylesterase; Fusarium mycotoxins; Cereals; Mycotoxin analysis
The selective enzymatic deacetylation of T-2 toxin to give HT-2 toxin has been investigated in aqueous crude extracts of different cereals and exploited to develop an analytical method for the determination of the sum of T-2 and HT-2 toxins. The method has been validated for the analysis of total T-2 and HT-2 toxins in maize, wheat, and oats, showing recoveries from 72 to 97% for maize, from 67 to 84% for wheat, and from 61% to 87% for oats, at spiking levels of 20-400 mu g/kg, with relative standard deviation lower than 10%. Liquid chromatography-tandem mass spectrometry was used for quantitative toxin determination. The potential biological role of this enzymatic conversion and its perspectives for application in the development of antibody-based analytical techniques are discussed.
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