期刊
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
卷 394, 期 2, 页码 507-512出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-009-2722-z
关键词
Gold nanoparticles; Homogeneous immunoassay; Human Serum Albumin in urine; Visible absorption detection
A sensitive homogeneous immunoassay, using human serum albumin (HSA) as a model analyte coupled with simple visible absorption detection, has been developed. The new assay is based on the use of gold nanoparticles functionalized with the target protein, which compete with the analyte for the binding of a specific polyclonal antibody. The binding of antibodies to the functionalized nanoparticles determines a shift of the visible absorption maximum of the gold colloid, and quantification of the analyte could be obtained as the competitive inhibition of the binding of antibodies to the nanoparticles. The proposed immunoassay has been optimized and successfully applied to measuring HSA in human urine samples, in which results agreed well with those obtained by a nephelometric reference method.
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