期刊
CONSERVATION GENETICS
卷 8, 期 4, 页码 983-985出版社
SPRINGER
DOI: 10.1007/s10592-006-9207-9
关键词
Stegastes partitus; Stegastes leucostictus; Stegastes planifrons; Stegastes adustus; Microspathodon chrysurus
Fourteen primer pairs were designed from 47 sequences containing microsatellites isolated from a genomic library enriched for (GACA)(4) in Stegastes partitus. Seven of these were shown to be polymorphic and provided clean amplification products. These seven loci were further characterized using 38 individuals, yielding a range in number of alleles from 5 to 31 and observed and expected heterozygosity values ranging from 0.45-0.89 and 0.66-0.96 respectively. Six of seven loci conformed to Hardy-Weinberg equilibrium. Successful amplification was also achieved in other Pomacentridae.
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