期刊
DEVELOPMENTAL DYNAMICS
卷 236, 期 8, 页码 2172-2180出版社
WILEY
DOI: 10.1002/dvdy.21211
关键词
miRNA; microarray; notch; hedgehog; cyclopamine; DAPT
资金
- NIGMS NIH HHS [T32 GM 008554, GM62758, GM 075790, T32 GM008554] Funding Source: Medline
microRNAs (miRNAs) are small (similar to 22 nucleotide) non-coding RNAs that regulate gene expression at the post-transcriptional level, typically by inhibiting translation. The genes encoding these small RNAs are estimated to comprise approximately 2-3% of animal genomes yet potentially regulate a majority of protein-coding genes including those involved in cell specification and development. A key remaining question is to identify target mRNAs regulated by microRNAs. As a means to identify potential targets, we designed a sensitive microarray to analyze global miRNA expression patterns at twelve developmental stages in zebrafish. Further, we conducted arrays on zebrafish embryos treated with small molecule inhibitors of the Hedgehog and Notch signaling pathways to enable identification of differentially expressed miRNAs that target genes controlling key developmental pathways during early embryogenesis.
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