期刊
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
卷 395, 期 7, 页码 2377-2385出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-009-3118-9
关键词
Fe3O4/SiO2 magnetic nanoparticles; Immobilized enzyme; Hydrogen peroxide; Glucose; Fluorometry; Catalysts; Clinical/biomedical analysis
资金
- National Science Foundation of China [20877031, 20677019]
Sensitive fluorescent probes for the determination of hydrogen peroxide and glucose were developed by immobilizing enzyme horseradish peroxidase (HRP) on Fe3O4/SiO2 magnetic core-shell nanoparticles in the presence of glutaraldehyde. Besides its excellent catalytic activity, the immobilized enzyme could be easily and completely recovered by a magnetic separation, and the recovered HRP-immobilized Fe3O4/SiO2 nanoparticles were able to be used repeatedly as catalysts without deactivation. The HRP-immobilized nanoparticles were able to activate hydrogen peroxide (H2O2), which oxidized non-fluorescent 3-(4-hydroxyphenyl)propionic acid to a fluorescent product with an emission maximum at 409 nm. Under optimized conditions, a linear calibration curve was obtained over the H2O2 concentrations ranging from 5.0 x 10(-9) to 1.0 x 10(-5) mol L-1, with a detection limit of 2.1 x 10(-9) mol L-1. By simultaneously using glucose oxidase and HRP-immobilized Fe3O4/SiO2 nanoparticles, a sensitive and selective analytical method for the glucose detection was established. The fluorescence intensity of the product responded well linearly to glucose concentration in the range from 5.0 x 10(-8) to 5.0 x 10(-5) mol L-1 with a detection limit of 1.8 x 10(-8) mol L-1. The proposed method was successfully applied for the determination of glucose in human serum sample.
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