期刊
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
卷 392, 期 1-2, 页码 305-312出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-008-2231-5
关键词
chemical cross-linking; amine-reactive cross-linkers; mass spectrometry
资金
- Deutsche Forschungsgemeinschaft (DFG) [SI 867/7-1]
- University of Leipzig
- Martin Luther University Halle-Wittenberg
In this report we summarize our experiences with the reaction products of N-hydroxysuccinimide (NHS) esters, which are widely used for chemical cross-linking of lysine residues in proteins. We describe the products, which should be scrutinized during data analysis using customized software when NHS esters are employed for chemical cross-linking. Reaction products of NHS esters were observed not only with lysines, but also with serines, tyrosines, and threonines. This report is intended to be a practical guide for those working in the field of chemical cross-linking and mass spectrometry.
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