期刊
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
卷 388, 期 8, 页码 1717-1724出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-007-1399-4
关键词
comprehensive two-dimensional high-performance liquid chromatography; reversed phase separation; enantioselective separation; 3,5-dinitrobenzoyl amino acids; chiral stationary phase; O-9-(tert-butylcarbamoyl) quinidine; nonporous silica; sub-2-mu m particles
A feasibility study on the fast enantioselective two-dimensional HPLC separation of racemic amino acid derivatives is presented. The method involves the on-line coupling of a narrow-bore C18 RP column in the first dimension to a short enantioselective column based on nonporous 1.5 mu m particles modified with quinidine carbamate as chiral selector in the second dimension. Conceptually, the system was designed to enable both time-controlled repeated transfer of fractions of the eluate and detector-controlled transfer of selected fractions from column 1 to column 2. To avoid volume overloading of the second chiral column, a narrow-bore reversed phase column was installed in the first dimension. Due to the fast (less than 1.5 minutes) enantiomer separation that occurs in the second dimension, the overall analysis time for the two-dimensional separation of a mixture of nine racemic 3,5-dinitrobenzoyl amino acids was optimized at 16 minutes.
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