期刊
PLOS GENETICS
卷 3, 期 8, 页码 1377-1388出版社
PUBLIC LIBRARY SCIENCE
DOI: 10.1371/journal.pgen.0030136
关键词
-
资金
- Intramural NIH HHS Funding Source: Medline
- NHGRI NIH HHS [HG03110, K22 HG003169, HG003169, R01 HG003110] Funding Source: Medline
The identification of regulatory elements from different cell types is necessary for understanding the mechanisms controlling cell type-specific and housekeeping gene expression. Mapping DNasel hypersensitive ( HS) sites is an accurate method for identifying the location of functional regulatory elements. We used a high throughput method called DNase-chip to identify 3,904 DNasel HS sites from six cell types across 1% of the human genome. A significant number ( 22%) of DNasel HS sites from each cell type are ubiquitously present among all cell types studied. Surprisingly, nearly all of these ubiquitous DNasel HS sites correspond to either promoters or insulator elements: 86% of them are located near annotated transcription start sites and 10% are bound by CTCF, a protein with known enhancer-blocking insulator activity. We also identified a large number of DNasel HS sites that are cell type specific ( only present in one cell type); these regions are enriched for enhancer elements and correlate with cell type-specific gene expression as well as cell type-specific histone modifications. Finally, we found that approximately 8% of the genome overlaps a DNasel HS site in at least one the six cell lines studied, indicating that a significant percentage of the genome is potentially functional.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据