期刊
CEREBRAL CORTEX
卷 17, 期 8, 页码 1782-1787出版社
OXFORD UNIV PRESS INC
DOI: 10.1093/cercor/bhl087
关键词
extrasynaptic GABA(A) receptor; single-cell RT-PCR; spillover; tonic inhibition; alpha 5 subunit
Fast inhibitory synaptic transmission is primarily mediated by synaptically released gamma-aminobutyric acid (GABA) acting on postsynaptic GABA(A) receptors. GABA acting on GABA(A) receptors produces not only phasic but also tonic inhibitions by persistent activation of extrasynaptic receptors. However, the mechanistic characteristics of tonic inhibition in the neoconex are not well-understood. To address this, we studied pharmacologically isolated GABA(A) receptor-mediated currents in neocortical pyramidal neurons in rat brain slices. Bath application of bicuculline blocked miniature inhibitory postsynaptic currents (mIPSCs) and produced an outward shift in baseline holding current (I-hold). Low concentrations of SR95531, a competitive GABA(A) receptor antagonist, abolished mIPSCs but had no significant effect on I-hold. The benzodiazepine midazolam produced an inward shift in I-hold by augmenting tonic GABA(A) receptor-mediated currents, which were significantly greater in layer V neurons than in layer II/III. Single-cell reverse transcriptase-polymerase chain reaction (RT-PCR) revealed a relatively higher expressions of alpha 1 and alpha 5 subunit mRNA in layer V neurons. L-655708, an alpha 5 subunit-specific inverse agonist, reduced tonic currents in layer V but not in layer II/III neurons, whereas zolpidem, an alpha 1-subunit agonist, exerted equivalent effects in both layers. These data suggest that the alpha 1 GABA(A) receptor subunit is generally involved in tonic inhibition in pyramidal neurons of the neocortex, whereas the alpha 5 subunit is specifically involved in layer V neurons.
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