期刊
MOLECULAR AND CELLULAR BIOLOGY
卷 27, 期 16, 页码 5639-5649出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/MCB.00418-07
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资金
- NCI NIH HHS [K22 CA100017] Funding Source: Medline
- NIEHS NIH HHS [P30 ES007784, ES07784] Funding Source: Medline
- NIGMS NIH HHS [R01 GM062631, GM62631] Funding Source: Medline
ATP-dependent chromatin remodeling complexes have been implicated in the regulation of transcription, replication, and more recently DNA double-strand break repair. Here we report that the Ies3p subunit of the Saccharomyces cerevisiae INO80 chromatin remodeling complex interacts with a conserved tetratricopeptide repeat domain of the telomerase protein Est1p. Deletion of IES3 and some other subunits of the complex induced telomere elongation and altered telomere position effect. In telomerase-negative mutants, loss of Ies3p delayed the emergence of recombinational survivors and stimulated the formation of extrachromosomal telomeric circles in survivors. Deletion of IES3 also resulted in heightened levels of telomere-telomere fusions in telomerase-deficient strains. In addition, a delay in survivor formation was observed in an Arp8p-deficient mutant. Because Arp8p is required for the chromatin remodeling activity of the INO80 complex, the complex may promote recombinational telomere maintenance by altering chromatin structure. Consistent with this notion, we observed preferential localization of multiple subunits of the INO80 complex to telomeres. Our results reveal novel functions for a subunit of the telomerase complex and the INO80 chromatin remodeling complex.
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