期刊
ANALYTICA CHIMICA ACTA
卷 1046, 期 -, 页码 48-68出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.aca.2018.09.021
关键词
Monolith; Monolithic column; Intact protein separation; Liquid chromatography
资金
- Oklahoma Center for the Advancement of Science and Technology [AR17-022]
In this article we survey 256 references (with an emphasis on the papers published in the past decade) on monolithic columns for intact protein separation. Protein enrichment and purification are included in the broadly defined separation. After a brief introduction, we describe the types of monolithic columns and modes of chromatographic separations employed for protein separations. While the majority of the work is still in the research and development phase, papers have been published toward utilizing monolithic columns for practical applications. We survey these papers as well in this review. Characteristics of selected methods along with their pros and cons will also be discussed. (C) 2018 Elsevier B.V. All rights reserved.
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