期刊
ANALYTICA CHIMICA ACTA
卷 1042, 期 -, 页码 37-43出版社
ELSEVIER
DOI: 10.1016/j.aca.2018.08.020
关键词
HPV; Cervical cancer; Genomagnetic assay; Electrode chip; LAMP amplification
资金
- Czech Science Foundation [P206/12/G151, 17-08971S, MEYS - NPS I - LO1413]
- MH CZ - DRO (MMCI) [00209805]
Major cause of cervical cancer is a persistent infection with high-risk types of human papillomaviruses (HPV). For that reason, HPV testing is now becoming an important addition to standard cytological screening of cervical malignancies in women (known as Pap test). New methods are sought which could offer rapid and inexpensive detection schemes, such as those based on electrochemical (EC) readout. Here, we developed an assay for parallel detection of two most oncogenic high-risk HPV types, HPV16 and HPV18, by combining loop-mediated amplification (LAMP) of template DNA, its separation using magnetic beads and detection with amperometry at carbon-based electrode chips. Our EC-LAMP test enabled us to successfully discriminate both HPV types not only in cancer cell lines, but also using clinical material obtained from HPV-positive patient samples. (C 2018 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据