期刊
ANALYTICA CHIMICA ACTA
卷 846, 期 -, 页码 44-50出版社
ELSEVIER
DOI: 10.1016/j.aca.2014.07.024
关键词
Electrochemical biosensor; InvA gene; Salmonella; Rolling circle amplification; Gold nanoparticles
资金
- National Natural Science Foundation of China [21075141, 81101638]
- Science and Technology Plan Project of Yuzhong District of Chongqing [20120212]
- Natural Science Foundation [CSTC2013jjB10019]
A novel electrochemical sensing strategy was developed for ultrasensitive and rapid detection of Salmonella by combining the rolling circle amplification with DNA-AuNPs probe. The target DNA could be specifically captured by probe 1 on the sensing interface. Then the circularization mixture was added to form a typical sandwich structure. In the presence of dNTPs and phi29 DNA polymerase, the RCA was initiated to produce micrometer-long single-strand DNA. Finally, the detection probe (DNA-AuNPs) could recognize RCA product to produce enzymatic electrochemical signal. Under optimal conditions, the calibration curve of synthetic target DNA had good linearity from 10 aM to 10 pM with a detection limit of 6.76 aM (S/N = 3). The developed method had been successfully applied to detect Salmonella as low as 6 CFU mL(-1) in real milk sample. This proposed strategy showed great potential for clinical diagnosis, food safety and environmental monitoring. (C) 2014 Elsevier B.V. All rights reserved.
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