期刊
ANALYTICA CHIMICA ACTA
卷 848, 期 -, 页码 51-60出版社
ELSEVIER
DOI: 10.1016/j.aca.2014.08.001
关键词
Desorption electrospray ionization mass spectrometry; Porcine oocyte; In vitro maturation; Data fusion; Principal component analysis
资金
- German Academic Exchange Service (DAAD)
- Fundacion Gran Mariscal de Ayacucho (FUNDAYACUCHO)
- Foundation L'Oreal/UNESCO for Women in Science (Italian Award L'Oreal/UNESCO for Women in Science)
- Purdue University Center for Cancer Research Small Grants
- Brazilian National Council for Scientific and Technological Development [CNPq 237237/2012-1]
- NIH
- [1R21EB015722]
The development of sensitive measurements to analyze individual cells is of relevance to elucidate specialized roles or metabolic functions of each cell under physiological and pathological conditions. Lipids play multiple and critical roles in cellular functions and the application of analytical methods in the lipidomics area is of increasing interest. In this work, in vitro maturation of porcine oocytes was studied. Two independent sources of chemical information (represented by mass spectra in the positive and negative ion modes) from single oocytes (immature oocytes, 24-h and 44-h in vitro matured oocytes) were acquired by using desorption electrospray ionization-mass spectrometry (DESI-MS). Low and mid-level data fusion strategies are presented with the aim of better exploring the large amount of chemical information contained in the two mass spectrometric lipid profiles. Data were explored by principal component analysis (PCA) within the two multi-block approaches to include information on free fatty acids, phospholipids, cholesterol-related molecules, di- and triacylglycerols. After data fusion, clearer differences among immature and in vitro matured porcine oocytes were observed, which provide novel information regarding lipid metabolism throughout oocyte maturation. In particular, changes in TAG composition, as well as increase in fatty acid metabolism and membrane complexity were evidenced during the in vitro maturation process. This information can assist the improvement of in vitro embryo production for porcine species. (C) 2014 Elsevier B.V. All rights reserved.
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