期刊
ANALYTICA CHIMICA ACTA
卷 848, 期 -, 页码 80-87出版社
ELSEVIER
DOI: 10.1016/j.aca.2014.07.029
关键词
Listeria monocytogenes; Enzyme substrates; Food; Headspace solid-phase microextraction gas chromatography-mass spectrometry; Volatile organic compounds
资金
- bioMerieux S.A.
The rapid detection of Listeria monocytogenes contamination in food is essential to prevent food-borne illness in humans. The aim of this study was to differentiate non-contaminated milk from milk contaminated with L. monocytogenes using enzyme substrates coupled with the analysis of volatile organic compounds (VOCs). The method is based on the activity of beta-glucosidase and hippuricase enzymes and the detection of a specific VOC i.e. 2-nitrophenol and 3-fluoroaniline, respectively. VOCs were extracted, separated and detected by headspace-solid phase microextraction coupled to gas chromatography-mass spectrometry (HS-SPME GC-MS). This approach required the inclusion of the selective agent's cycloheximide, nalidixic acid and acriflavine HCl in the growth medium to inhibit interfering bacteria. The VOCs were liberated by L. monocytogenes provided that samples contained at least 1-1.5 x 10(2) CFU ml(-1) of milk prior to overnight incubation. This approach shows potential for future development as a rapid method for the detection of L. monocytogenes contaminated milk. (C) 2014 The Authors. Published by Elsevier B.V. All rights reserved. This is an open access article under the CC BY-NC-SA license.
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