期刊
ANALYTICA CHIMICA ACTA
卷 810, 期 -, 页码 10-16出版社
ELSEVIER
DOI: 10.1016/j.aca.2013.12.010
关键词
DNA based sensor; Gold amalgamation; Electrochemistry; Mercury ion; Cycling signal amplification
资金
- National 973 National Basic Research Program of China [2010CB732403]
- Research Fund for the National Science Foundation of Fujian Province [2011106003]
- Doctoral Program of Higher Education of China [20103514120003]
- National Natural Science Foundation of China [21075019, 21001033, 41176079]
- Program for Changjiang Scholars and Innovative Research Team in University [IRT1116]
Heavy metal ion pollution poses severe risks in human health and environmental pollutant, because of the likelihood of bioaccumulation and toxicity. Driven by the requirement to monitor trace-level mercury ion (Hg21, herein we construct a new DNA-based sensor for sensitive electrochemical monitoring of Hg2' by coupling target-induced formation of gold amalgamation on DNA-based sensing platform with gold amalgamation-catalyzed cycling signal amplification strategy. The sensor was simply prepared by covalent conjugation of aminated poly-T(25) oligonucleotide onto the glassy carbon electrode by typical carbodiimide coupling. Upon introduction of target analyte, Hg2+ ion was intercalated into the DNA polyion complex membrane based on T-Hg2+-T coordination chemistry. The chelated Hg2+ ion could induce the formation of gold amalgamation, which could catalyze the p-nitrophenol with the aid of NaBH4 and Ru(NH3)63' for cycling signal amplification. Experimental results indicated that the electronic signal of our system increased with the increasing Hg2+ level in the sample, and has a detection limit of 0.02 nM with a dynamic range of up to 1000 nM Hg2'. The strategy afforded exquisite selectivity for Hg2+ against other environmentally related metal ions. In addition, the methodology was evaluated for the analysis of Hg2+ in spiked tap-water samples, and the recovery was 87.9-113.8%. (C) 2013 Elsevier B.V. All rights reserved.
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