期刊
ANALYTICA CHIMICA ACTA
卷 852, 期 -, 页码 174-180出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.aca.2014.08.050
关键词
Carbon dots; Tryptophan; Peroxynitrite; Sensing; Fluorescence quenching
资金
- Fundacao para a Ciencia e Tecnologia (FCT, Lisbon) [SFRH/BD/81074/2011]
- Fundação para a Ciência e a Tecnologia [SFRH/BD/81074/2011] Funding Source: FCT
Tryptophan doped carbon dots (Trp-CD) were microwave synthesized. The optimum conditions of synthesizing of the Trp-CD were established by response surface multivariate optimization methodologies and were the following: 2.5 g of glucose and 300 mg of tryptophan diluted in 15 mL of water exposed for 5 min to a microwave radiation of 700 W. Trp-CD have an average size of 20 nm, were fluorescent with a quantum yield of 12.4% and the presence of peroxynitrite anion (ONOO ) provokes quenching of the fluorescence. The evaluated analytical methodology for ONOO detection shows a linear response range from 5 to 25 mu M with a limit of detection of 1.5 mu M and quantification of 4.9 mu M. The capability of the ONOO quantification was evaluated in standard solutions and in fortified serum samples. (C) 2014 Elsevier B. V. All rights reserved.
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