期刊
ANALYTICA CHIMICA ACTA
卷 788, 期 -, 页码 129-134出版社
ELSEVIER
DOI: 10.1016/j.aca.2013.05.063
关键词
Signal amplification; Glycoprotein enrichment; Magnetic microbeads; Gold nanoparticle; Mass spectrometry
资金
- National Science and Technology Key Project of China [2012CB910602, 2012AA020203, 2012CB910103]
- National Natural Science Foundation of China [21025519, 31070732]
- Shanghai Projects [11XD1400800, B109]
We describe a novel method for rapid and ultrasensitive detection of intact glycoproteins without enzymatic pretreatment which was commonly used in proteomic research. This method is based on using gold nanoparticle (AuNP) as signal tag in laser desorption/ionization mass spectrometry (LDI-MS) analysis combined with boronic acid assisted isolation strategy. Briefly speaking, target glycoproteins were firstly isolated from sample solution with boronic acid functionalized magnetic microparticles, and then the surface modified gold nanoparticles were added to covalently bind to the glycoproteins. After that, these AuNP tagged glycoproteins were eluted from magnetic microparticles and applied to LDI-MS analysis. The mass signal of AuNP rather than that of glycoprotein was detected and recorded in this strategy. Through data processing of different standard glycoproteins, we have demonstrated that the signal of AuNP could be used to quantitatively represent glycoprotein. This method allows femtomolar detection of intact glycoproteins. We believe that the successful validation of this method on three different kinds of glycoproteins suggests the potential use for tracking trace amount of target glycoproteins in real biological samples in the near future. (C) 2013 Elsevier B.V. All rights reserved.
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