期刊
ANALYTICA CHIMICA ACTA
卷 804, 期 -, 页码 313-320出版社
ELSEVIER
DOI: 10.1016/j.aca.2013.10.037
关键词
Anion effect; Capillary electrochromatography; Ionic liquid; Monolithic column; Protein separation
资金
- Ministry of Science and Technology of China [2012AA101609]
- National Natural Science Foundation of China [21075089]
- Ministry of Science and Technology of Tianjin [10SYSYJC28300]
- Program for Changjiang Scholars and Innovative Research Team in University [IRT1166]
A series of ionic liquids (ILs) monolithic capillary columns based on 1-vinyl-3-octylimidazolium (ViOcIm(+) were prepared by two approaches (one-pot approach and anion-exchange approach). The effects of different anions (bromide, Br-; tetrafluoroborate, BF4-; hexafluorophosphate, PF6-; and bis-trifluoromethanesulfonylimide, NTf2-) on chromatography performance of all the resulting columns were investigated systematically under capillary electrochromatography (CEC) mode. The results indicated that all these columns could generate a stable reversed electroosmotic flow (EOF) over a wide pH range from 2.0 to 12.0. For the columns prepared by one-pot approach, the EOF decreased in the order of ViOcIm(+)Br(-) > ViOcIm(+)BF(4)(-) > ViOcIm(+)PF(6)(-) > ViOcIm(+)NTf(2)(-) under the same CEC conditions; the ViOcIm+Br- based column exhibited highest column efficiencies for the test small molecules; the ViOcIm(+)NTf(2)(-) based column possessed the strongest retention for aromatic hydrocarbons; and baseline separation of four standard proteins was achieved on ViOcIm(+)NTf(2)(-) based column corresponding to the highest column efficiency of 479 000 N m(-1) for cytochrome c (Cyt c). These results indicated that the property of ILs based columns could be tuned successfully by changing anions, which gave these columns potential to separate both small molecules and macro biomolecules. Crown Copyright (C) 2013 Published by Elsevier B.V. All rights reserved.
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