期刊
EMBO JOURNAL
卷 26, 期 15, 页码 3720-3728出版社
WILEY
DOI: 10.1038/sj.emboj.7601786
关键词
bacteriophage SPP1; electron microscopy; Siphoviridae; signal transmission; structural analysis
资金
- Biotechnology and Biological Sciences Research Council [B17360] Funding Source: researchfish
The majority of known bacteriophages have long noncontractile tails (Siphoviridae) that serve as a pipeline for genome delivery into the host cytoplasm. The tail extremity distal from the phage head is an adsorption device that recognises the bacterial receptor at the host cell surface. This interaction generates a signal transmitted to the head that leads to DNA release. We have determined structures of the bacteriophage SPP1 tail before and after DNA ejection. The results reveal extensive structural rearrangements in the internal wall of the tail tube. We propose that the adsorption device-receptor interaction triggers a conformational switch that is propagated as a domino-like cascade along the 1600 angstrom-long helical tail structure to reach the head-to-tail connector. This leads to opening of the connector culminating in DNA exit from the head into the host cell through the tail tube.
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