期刊
ANALYTICA CHIMICA ACTA
卷 784, 期 -, 页码 53-58出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.aca.2013.04.038
关键词
DNA-templated gold/silver nanoclusters (DNA-Au/Ag NCs); Deoxyribonuclease I (DNase I); Quenching; High performance liquid chromatography (HPLC)
资金
- National Natural Science Foundation of China [31100981]
- Research Fund for the Doctoral Program of Higher Education of China [20110182120014]
- Science Foundation of Southwest University [SWU110022]
Herein, fluorescent DNA-templated gold/silver nanoclusters (DNA-Au/Ag NCs) are presented as a novel probe for sensitive detection of deoxyribonuclease I (DNase I). The procedure is based on quenching fluorescence of DNA-Au/Ag NCs by DNase I digestion of the DNA (5'-CCCTFAATCCCC-3') template. This decrease in fluorescence intensity permitted sensitive detection of DNase I in a linear range of 0.013-60 mu gmL(-1), with a detection limit of 3 ng mL(-1) at a signal-to-noise ratio of 3. Furthermore, the practicality of this probe for detection of DNase I in human serum and saliva samples was validated, demonstrating its advantages of simplicity, selectivity, sensitivity and low cost. Importantly, satisfactory agreement between results obtained by the fluorescent method described here and high performance liquid chromatography (HPLC) further confirmed the reliability and accuracy of this approach. Crown Copyright (C) 2013 Published by Elsevier B.V. All rights reserved.
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