期刊
JOURNAL OF CHROMATOGRAPHY A
卷 1160, 期 1-2, 页码 64-70出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.chroma.2007.05.036
关键词
pressurized fluid extraction (PFE); soxhlet; sterols; sediment; Korea
In order to determine steroid compounds in GC/MS an analytical method using pressurized fluid extraction (PIE) was developed. While extracting in-house reference material (coastal sediment) typical recovery in PFE ranged from 80 to 120% (+/- 2.5-14.5) and the average extraction yield in PFE in comparison to conventional soxhlet extraction was 115%. In particular, the PFE showed higher extraction efficiency for C-29 and then sterols. Optimizing parameters such as temperature and pressure is critical in achieving this efficiency. Sterols in the sediment were derivatized with silyl reagent BSTFA in acetone for the final determination. A short column florisil cleanup offered the best separation of the GC/MS sensitive derivatives from co-contaminants. Thirty-three coastal sediment samples were analyzed using PFE and Soxhlet extraction methods. The results on extraction efficiency, silyl derivatization kinetics and purification efficiency demonstrated that PIE is far superior in extracting sterols from sediment samples. It is simple, fast, efficient and amenable for automation. (c) 2007 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据