期刊
ANALYTICA CHIMICA ACTA
卷 787, 期 -, 页码 203-210出版社
ELSEVIER
DOI: 10.1016/j.aca.2013.05.024
关键词
Codeine; Aptamer; Systematic evolution of ligands by exponential enrichment; Electrochemical aptasensor; Au-mesoporous silica nanoparticles
资金
- National Natural Science Foundation of China [20865006, 21165023]
- Program for Changjiang Scholars and Innovative Research Team in University, PCSIRT
In order to develop a sensor for opium alkaloid codeine detection, DNA aptamers against codeine were generated by SELEX (systematic evolution of ligands by exponential enrichment) technique. An aptamer HL7-14, which is a 37-mer sequence with K-d values of 0.91 mu M, was optimized by the truncation-mutation assay. The specificity investigation shows that HL7-14 exhibits high specificity to codeine over morphine, and almost cannot bind to other small molecule. With this new selected aptamer, a novel electrochemical label-free codeine aptamer biosensor based on Au-mesoporous silica nanoparticles (Au-MSN) as immobilized substrate has been proposed using [Fe(CN)(6)](3-/4-) as electroactive redox probe. The linear range covered from 10 pM to 100 nM with correlation coefficient of 0.9979 and the detection limit was 3 pM. Our study demonstrates that the biosensor has good specificity, stability and well regeneration. It can be used to detect codeine. (C) 2013 Elsevier B.V. All rights reserved.
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