期刊
ANALYTICA CHIMICA ACTA
卷 771, 期 -, 页码 65-72出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.aca.2013.02.004
关键词
Liquid chromatography-tandem mass spectrometry; Derivatization; High-throughput; Neurotransmitter; Brain microdialysis; Rat; Striatum
资金
- Hessia LOEWE program NeFF
- Hessia LOEWE program OSF
- European COST [BM0806, CM1007, CM1103]
We have developed and validated a sensitive method for the simultaneous determination of some monoamine neurotransmitters like dopamine (DA), norepinephrine (NE) and serotonin (5-HT) in rat brain microdialysate using high-performance liquid chromatography coupled to tandem mass spectrometry (LC/MS/MS). Sensitivity enhancement has been achieved by amine derivatization with the reagent (5-N-succinimidoxy-5-oxopentyl)triphenylphosphonium bromide (SPTPP) under mild conditions. The use of the selected reaction monitoring (SRM) mode has allowed detection of the analytes at a concentration of 30 pM (lower limit of quantification, LLOQ signal-to-noise ratio higher than 5) with an, accuracy of <= 3.80% and a precision of +/- 7.39 (%CV) for all neurotransmitters. Derivatization improves resolution and chromatographic retention times (3 min) by lipophilization. Linearity has been good (R>0.99) over a large concentration range (30-50,000 pM). The intra and inter-batch accuracy and precision were not greater than 4.8% and 6.4%, respectively. Therefore, the method was successfully applied for monitoring the concentration changes of neurotransmitters in microdialysis samples deriving from striatum rat brain region after amphetamine administration (3 mg kg(-1), i.p.). (C) 2013 Elsevier B.V. All rights reserved.
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