期刊
JOURNAL OF CELL BIOLOGY
卷 178, 期 4, 页码 583-594出版社
ROCKEFELLER UNIV PRESS
DOI: 10.1083/jcb.200610112
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- NICHD NIH HHS [HD40182, R01 HD040182] Funding Source: Medline
NudE and l are related proteins that interact with cytoplasmic dynein and LIS1. Their functional relationship and involvement in LIS1 and dynein regulation are not completely understood. We find that NudE and l each localize to mitotic kinetochores before dynein, dynactin, ZW10, and LIS1 and exhibit additional temporal and spatial differences in distribution from the motor protein. Inhibition of NudE and l caused metaphase arrest with misoriented chromosomes and defective microtubule attachment. Dynein and dynactin were both displaced from kinetochores by the injection of an anti-NudE/NudEL antibody. Dynein but not dynactin interacted with NudE surprisingly through the dynein intermediate and light chains but not the motor domain. Together, these results identify a common function for NudE and l in mitotic progression and identify an alternative mechanism for dynein recruitment to and regulation at kinetochores.
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