期刊
ANALYTICA CHIMICA ACTA
卷 790, 期 -, 页码 24-30出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.aca.2013.06.025
关键词
Cascade catalysis; Multi-functionalized magnetic graphene sphere; Cytochrome c; Glucose oxidase; Hybridization chain reaction; Electrochemical immunosensor
资金
- NNSF of China [21275119, 21105081, 21075100]
- Research Fund for the Doctoral Program of Higher Education (RFDP) [20110182120010]
- Ministry of Education of China [708073]
- Specialized Research Fund for the Doctoral Program of Higher Education [20100182110015]
- Natural Science Foundation Project of Chongqing City [CSTC-2010BB4121, CSTC-2009BA1003]
- Fundamental Research Funds for the Central Universities, China [XDJK2010C062, XDJK2012A004]
This paper constructed a reusable electrochemical immunosensor for the detection of thyroxine at an ultralow concentration using cascade catalysis of cytochrome c (Cyt c) and glucose oxidase (GOx) as signal amplified enhancer. It is worth pointing out that numerous Cyt c and GOx were firstly carried onto the double-stranded DNA polymers based on hybridization chain reaction (HCR), and then the amplified responses could be achieved by cascade catalysis of Cyt c and GOx recycling with the help of glucose. Moreover, multi-functionalized magnetic graphene sphere was synthesized and used as signal tag, which not only exhibited good mechanical properties, large surface area and an excellent electron transfer rate of graphene, but also possessed excellent redox activity and desirable magnetic property. With a sandwich-type immunoreaction, the proposed cascade catalysis amplification strategy could greatly enhance the sensitivity for the detection of thyroxine. Under the optimal conditions, the immunosensor showed a wide linear ranged from 0.05 pg mL(-1) to 5 ng mL(-1) and a low detection limit down to 15 fg mL(-1). Importantly, the proposed method offers promise for reproducible and cost-effective analysis of biological samples. (c) 2013 Elsevier B.V. All rights reserved.
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