期刊
ANALYTICA CHIMICA ACTA
卷 758, 期 -, 页码 66-71出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.aca.2012.10.050
关键词
Superoxide anion; Superoxide dismutase; Gold nanoparticles; Ionic liquid; Ultrasonic electrodeposition
资金
- National Natural Science Foundation of China [20875023, 51173038]
- Ph.D. Programs Foundation of the Ministry of Education of China [20114208130001]
- Natural Science Fund for Creative Research Groups of Hubei Province of China [2011CDA111]
- Foundation for Innovative Research Groups of the Education Department of Hubei Province [T201101]
A novel superoxide anion (O-2(center dot-)) biosensor is proposed based on the immobilization of copper-zinc superoxide dismutase (SOD) in a gold nanoparticle-chitosan-ionic liquid (GNPs-CS-IL) biocomposite film. The SOD-based biosensor was constructed by one-step ultrasonic electrodeposition of GNP-CS-IL composite onto glassy carbon electrode (GCE), followed by immobilization of SOD on the modified electrode. Surface morphologies of a set of representative films were characterized by scanning electron microscopy. The electrochemical performance of the biosensor was evaluated by cyclic voltammetry and chronoamperometry. A pair of quasi-reversible redox peaks of SOD with a formal potential of 0.257 V was observed at SOD/GNPs-CS-IL/GCE in phosphate buffer solution (PBS, 0.1 M, pH 7.0). The effects of varying test conditions on the electrochemical behavior of the biosensor were investigated. Furthermore, several electrochemical parameters were calculated in detail. Based on the biomolecule recognition of the specific reactivity of SOD toward O-2(center dot-), the developed biosensor exhibited a fast amperometric response (<5 s), wide linear range (5.6-2.7 x 10(3) nM), low detection limit (1.7 nM), and excellent selectivity for the real-time measurement of O-2(center dot-). The proposed method is promising for estimating quantitatively the dynamic changes of O-2(center dot-) in biological systems. (C) 2012 Elsevier B.V. All rights reserved.
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