期刊
ANALYTICA CHIMICA ACTA
卷 762, 期 -, 页码 47-53出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.aca.2012.12.008
关键词
Mercury; Oligonucleotide probe; Electrocatalytic amplification; Electrochemical biosensor
资金
- Natural Science Foundation of Shandong Province [ZR2012BL14]
- National Natural Science Foundation of China [21175037]
Engineered nucleic acid probes containing recognition and signaling functions find growing interest in biosensor design. In this paper, we developed a novel electrochemical biosensor for sensitive and selective detecting of Hg2+ based on a bifunctional oligonucleotide signal probe combining a mercury-specific sequence and a G-quadruplex (G4) sequence. For constructing the electrochemical Hg2+ biosensor, a thiolated, mercury-specific oligonucleotide capture probe was first immobilized on gold electrode surface. In the presence of Hg2+, a bifunctional oligonucleotide signal probe was hybridized with the immobilized capture probe through thymine-mercury(II)-thymine interaction-mediated surface hybridization. The further interaction between G4 sequence of the signal probe and hemin generated a G4-hemin complex, which catalyzed the electrochemical reduction of hydrogen peroxide, producing amplified readout signals for Hg2+ interaction events. This electrochemical Hg2+ biosensor was highly sensitive and selective to Hg2+ in the concentration of 1.0 nM to 1 mu M with a detection limit of 0.5 nM. The new design of bifunctional oligonucleotide signal probes also provides a potential alternative for developing simple and effective electrochemical biosensors capable of detecting other metal ions specific to natural or artificial bases. (C) 2012 Elsevier B.V. All rights reserved.
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