期刊
ANALYTICA CHIMICA ACTA
卷 799, 期 -, 页码 44-50出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.aca.2013.08.012
关键词
Splitting aptamer; Pyrene dimer; gamma-Cyclodextrin; Adenosine triphosphate; Fluorescence lifetime
资金
- National Key Natural Science Foundation of China [21135001]
- 973 National Key Basic Research Program [2011CB911000]
- National Natural Science Foundation of China [21075032, 21005026]
A highly sensitive and selective fluorescence aptamer biosensors for the determination of adenosine triphosphate (ATP) was developed. Binding of a target with splitting aptamers labeled with pyrene molecules form stable pyrene dimer in the gamma-cyclodextrin (gamma-CD) cavity, yielding a strong excimer emission. We have found that inclusion of pyrene dimer in gamma-cyclodextrin cavity not only exhibits additive increases in quantum yield and emission lifetime of the excimer, but also facilitates target-induced fusion of the splitting aptamers to form the aptamer/target complex. As proof-of-principle, the approach was applied to fluorescence detection of adenosine triphosphate. With an anti-ATP aptamer, the approach exhibits excimer fluorescence response toward ATP with a maximum signal-to-background ratio of 32.1 and remarkably low detection limit of 80 nM ATP in buffer solution. Moreover, due to the additive fluorescence lifetime of excimer induced by gamma-cyclodextrin, time-resolved measurements could be conveniently used to detect as low as 0.5 mu M ATP in blood serum quantitatively. (c) 2013 Elsevier B.V. All rights reserved.
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