期刊
ANALYTICA CHIMICA ACTA
卷 769, 期 -, 页码 40-48出版社
ELSEVIER
DOI: 10.1016/j.aca.2013.01.038
关键词
Graphene oxide; Nano-carrier; Serum albumin; Fluorescence enhancement; Chemometrics; Biosensor
资金
- National Natural Science Foundation of China [NSFC-21065007]
- Jiangxi Provincial Education Department Science Foundation [GJJ10037]
- Graduate Student Innovation Program of Nanchang University [YC2012-S012]
- State Key Laboratory of Food Science and Technology of Nanchang University [SKLF-MB-201002, SKLF-TS-200919]
The interaction of graphene oxide (GO), a medicinal drug (10-hydroxy camptothecin (HCPT)), and bovine serum albumin (BSA) was investigated with the aim of developing a method for the analysis of serum albumin proteins. It was demonstrated that HCPT could be readily loaded onto GO via the, pi-pi stacking interaction, and the delivery of HCPT to BSA was improved in the presence of GO; this, in turn, facilitated the binding interaction of HCPT and BSA. Chemometrics methods, multivariate curve resolution-alternating least squares (MCR-ALS) and parallel factor analysis (PARAFAC), were applied to resolve spectral data, and this assisted in the elucidation of the above interaction. GO was found to enhance the fluorescence response of HCPT to BSA, and thus, a low cost fluorescence bio-sensing platform was developed for fluorescence-enhanced detection of BSA based on GO. The satisfactory analytical performance of this biosensor for BSA was attributed to the structure and electronic properties of GO. (C) 2013 Elsevier B.V. All rights reserved.
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