期刊
ANALYTICA CHIMICA ACTA
卷 778, 期 -, 页码 63-69出版社
ELSEVIER
DOI: 10.1016/j.aca.2013.03.059
关键词
Gold nanoparticles; Pyrocatechol violet; Antibiotics; Colorimetric; Hydrogen-bonding
资金
- Major National Scientific Research Plan of China (973 Program) [2011CB933202]
- National Natural Science Foundation of China [21205132]
- State Key Laboratory of Environmental Chemistry and Ecotoxicology [KF2010-23]
Antibiotics residues in foods are very harmful to human beings. Determination of antibiotics residues relies largely on the availability of adequate analytical techniques. Currently, there is an urgent need for on site and real time detection of antibiotics in food. In this work, a novel one step synthesis of gold nanoparticles (AuNPs) was proposed using pyrocatechol violet (PCV) as a reducer agent. Highly sensitive and selective colorimetric detection of four antibiotics kanamycin mono sulfate (KA), neomycin sulfate (NE), streptomycin sulfate (ST) and bleomycin sulfate (BL) was realized during the formation of AuNPs. PCV has OH groups and these antibiotics have -OH, -NH2, -NH- groups, so there may be some special hydrogen-bonding interactions between PCV and these antibiotics. Therefore, the presence of KA, NE, ST and BL would influence the synthesis of AuNPs, then the color and state of AuNPs would change, which could be observed with the naked eye or a UV-vis spectrophotometer. Results showed that A(670) was linear with the logarithm of KA concentration in the range from 1.0 x 10(-8) to 5.0 x 10(-7) M and 5.0 x 10-7 to 5.5 x 10(-5) M. The detection limit of KA was 1.0 x 10(-9) M (S/N = 3). The coexisting substances including 1.0 x 10-5 M phenylalanine, alanine, glycerol, glucose, Mg2+, Ca2+, Na+, K+, CO32-, SO42-, NO3-, Cl- and Br- did not affect the determination of 1.0 x 10(-7) M antibiotics. In particular, the proposed method could be applied successfully to the detection of antibiotics in the pretreated liquid milk products. (C) 2013 Elsevier B.V. All rights reserved.
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