期刊
ANALYTICA CHIMICA ACTA
卷 805, 期 -, 页码 95-100出版社
ELSEVIER
DOI: 10.1016/j.aca.2013.10.045
关键词
Bimetallic gold-silver nanoplate array; Localized surface plasmon resonance (LSPR); Surface enhanced Raman scattering (SERS); Streptavidin/biotin assemblies
资金
- Chinese 973 Project [2012CB933302]
- National Natural Science Foundation of China [21175022]
- Ministry of Science & Technology of China [2012AA022703]
The silver-modified gold nanoplate arrays as bimetallic surface-enhanced Raman scattering (SERS) substrates were optimized for the surface-enhanced Raman detection of streptavidin/biotin monolayer assemblies. The bimetallic gold-silver nanoplate arrays were fabricated by coating silver nanoparticles uniformly on the gold nanoplate arrays. Depending on silver nanoparticle coating, the localized surface plasmon resonance (LSPR) peak of the bimetallic gold-silver nanoplate arrays blue-shifted and broadened significantly. The common probe molecule, Niel Blue A sulfate (NBA) was used for testing the SERS activity of the bimetallic gold-silver nanoplate arrays. The SERS intensity increased with the silver nanoparticle coating, due to a large number of hot spots and nanoparticle interfaces. The platforms were tested against a monolayer of streptavidin functionalized over the bimetallic gold-silver nanoplate arrays showing that good quality spectra could be acquired with a short acquisition time. The supramolecular interaction between streptavidin (strep) and biotin showed subsequent modification of Raman spectra that implied a change of the secondary structure of the host biomolecule. And the detection concentration for biotin by this method was as low as 1.0 nM. The enhanced SERS performance of such bimetallic gold-silver nanoplate arrays could spur further interest in the integration of highly sensitive biosensors for rapid, nondestructive, and quantitative bioanalysis, particularly in microfluidics. (C) 2013 Published by Elsevier B.V.
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