期刊
ORGANIC LETTERS
卷 9, 期 17, 页码 3343-3346出版社
AMER CHEMICAL SOC
DOI: 10.1021/ol071301h
关键词
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资金
- NIGMS NIH HHS [R01 GM058822-01, GM58822, R01 GM058822] Funding Source: Medline
Lantibiotic synthetases generate intramolecular thioether cross-links within peptides through the Michael-type addition of cysteines onto dehydroamino acids originating from Ser and Thr. Presented here is an assay that readily distinguishes between enzymatic and nonenzymatic formation of these cross-links. The results demonstrate unequivocally that lacticin 481 synthetase can generate non-native thioether cross-links.
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