期刊
ANALYTICA CHIMICA ACTA
卷 753, 期 -, 页码 27-31出版社
ELSEVIER
DOI: 10.1016/j.aca.2012.09.036
关键词
Ochratoxin A detection; Aptamer; Electrochemical aptasensor; Ultrasensitive; One-step detection
资金
- Huangshan Young Scholar Fund of Hefei University of Technology [407-037025]
- Science and Technology Research Project of General Administration of Quality Supervision, Inspection and Quarantine of PR China [201210127]
- 12th Five Years Key Programs [2012BAK08B01, 2012BAK17B10, SS2012AA101001]
- National Science Foundation of China [20906039]
A one-step electrochemical aptasensor using the thiol- and methylene blue- (MB-) dual-labeled aptamer modified gold electrode for determination of ochratoxin A (OTA) was presented in this research. The aptamer against OTA was covalently immobilized on the surface of the electrode by the self-assembly effect and used as recognition probes for OTA detection by the binding induced folding of the aptamer. Under the optimal conditions, the developed electrochemical aptasensor demonstrated a wide linear range from 0.1 pg mL(-1) to 1000 pg mL(-1) with the limit of detection (LOD) of 0.095 pg mL(-1), which was an extraordinary sensitivity compared with other common methods for OTA detection. Moreover, as a practical application, this proposed electrochemical aptasensor was used to monitor the OTA level in red wine samples without any special pretreatment and with satisfactory results obtained. Study results showed that this electrochemical aptasensor could be a potential useful platform for on-site OTA measurement in real complex samples. (C) 2012 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据