期刊
ANALYTICA CHIMICA ACTA
卷 729, 期 -, 页码 62-66出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.aca.2012.03.050
关键词
Doxorubicin; Fluorescence lifetime; Self-referenced measurements
资金
- Spanish Ministerio de Educacion
Dual lifetime referencing (DLR) is introduced as a rapid and self-referenced method for measuring the concentration of a fluorescent analyte in solution. The fluorescent cancer chemotherapeutic doxorubicin was chosen as a medically relevant analyte and blended with a reference dye (Ru(dpp)(3)) that displays overlapping excitation and emission spectra. The relative contributions of the short-lived (nanoseconds) fluorescent analyte and the long-lived (microseconds) reference dye define the observed lifetime. Measuring this lifetime by both frequency-domain DLR and time-domain DLR yields similar analytical ranges and limits of detection (0.4 mu M). To assess the matrix effect of medical samples, the standard addition method was employed to both modes of DLR. Urine was spiked with doxorubicin and recovery rates of >= 97% were obtained. (C) 2012 Elsevier B.V. All rights reserved.
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