期刊
ANALYTICA CHIMICA ACTA
卷 744, 期 -, 页码 54-59出版社
ELSEVIER
DOI: 10.1016/j.aca.2012.07.011
关键词
Amino acids; Microwave-irradiation hydrolysis; Gas chromatography-mass spectrometry; Selenium enriched yeast
A simple, rapid microwave digestion procedure for protein hydrolysis preceding the determination of amino acids in yeast using gas chromatography-mass spectrometry (GC-MS) is described. Protein hydrolysis was performed in a focused microwave using 4 M methanesulfonic acid (MAS). Amino acids were derivatized with methyl chlorofomate (MCF) and extracted into chloroform prior to GC-MS analysis. The microwave parameters, including power, temperature and heating time, were optimized. It was found that temperature and heating time were the most influential factors. A total of 17 amino acids were determined in selenium-enriched yeast with use of standard addition calibration. Limits of detection and quantitation (LODs/LOQs) of the amino acids measured were in the sub-nmol range, suitable for monitoring of amino acids in yeast and other food products. (C) 2012 Published by Elsevier B.V.
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