期刊
ANALYTICA CHIMICA ACTA
卷 755, 期 -, 页码 46-53出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.aca.2012.10.004
关键词
Aptasensor; Functionalized graphene; Pd nanoparticles; Thrombin; Hemin/G-quadruplex
资金
- NNSF of China [21075100, 21105081]
- State Key Laboratory of Electroanalytical Chemistry [SKLEAC2010009]
- Fundamental Research Funds for the Central Universities [XDJK2012A004]
- Specialized Research Fund for the Doctoral Program of Higher Education [20100182110015]
- Ministry of Education of China [708073]
- Postgraduate Science and Technology Innovation Program of Southwest China University [KB2010006]
In this work, an advanced sandwich-type electrochemical aptasensor for thrombin was proposed by integrating hemin/G-quadruplex with functionalized graphene-Pd nanoparticles composites (PdNPs-RGs). The hemin/G-quadruplex formed by intercalating hemin into thrombin binding aptamer (TBA), firstly acted as a NADH oxidase, assisting the oxidation of NADH to NAD(+) accompanying with the generation of H2O2 in the presence of dissolved O-2. Subsequently, the hemin/G-quadruplex acted as HRP-mimicking DNAzyme that rapidly bioelectrocatalyze the reduction of the produced H2O2. At the same time, the Pd nanoparticles supported on p-iodoaniline functionalized graphene were also adopted to catalyze the reduction of H2O2. Thus, with the dual catalysis, a dramatically amplified electrochemical signal could be obtained. Besides, the avidin-biotin system for binding aptamer sequences on electrodes not only improved the sensitivity of thrombin analysis but also obtained an acceptable repeatability of the aptasensor. With several factors mentioned above, a wide linear ranged from 0.1 pM to 50 nM was acquired with a relatively low detection limit of 0.03 pM (defined as S/N=3). These excellent performances provided our approach a promising way for ultrasensitive assay in electrochemical aptasensors. (C) 2012 Elsevier B.A. All rights reserved.
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