期刊
ANALYTICA CHIMICA ACTA
卷 688, 期 2, 页码 99-103出版社
ELSEVIER
DOI: 10.1016/j.aca.2010.12.017
关键词
Aptamers; Multi-analyte detection; Quantum dots; Small molecules; Square wave voltammetry
资金
- National Natural Science Foundation of China [20905062, 20675064]
- Natural Science Foundation of Chongqing City [CSTC-2009BB5003]
- State Key Laboratory of Electroanalytical Chemistry [SKLEAC2010009]
- China Postdoctoral Science Foundation [20090460715, 201003305]
- Fundamental Research Funds for the Central Universities [XDJK2009B013]
- Southwest University [SWUB2008078]
A novel strategy for signal on and sensitive one-spot simultaneous detection of multiple small molecular analytes based on electrochemically encoded barcode quantum dot (QD) tags is described. The target analytes, adenosine triphosphate (ATP) and cocaine, respectively, are sandwiched between the corresponding set of surface-immobilized primary binding aptamers and the secondary binding aptamer/QD bioconjugates. The captured QDs yield distinct electrochemical signatures after acid dissolution, whose position and size reflect the identity and level, respectively, of the corresponding target analytes. Due to the inherent amplification feature of the QD labels and the signal on detection scheme, as well as the sensitive monitoring of the metal ions released upon acid dissolution of the QD labels, low detection limits of 30 nM and 50 nM were obtained for ATP and cocaine, respectively, in our assays. Our multi-analyte sensing system also shows high specificity to target analytes and promising applicability to complex sample matrix, which makes the proposed assay protocol an attractive route for screening of small molecules in clinical diagnosis. (C) 2010 Elsevier B.V. All rights reserved.
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