期刊
ANALYTICA CHIMICA ACTA
卷 687, 期 2, 页码 150-158出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.aca.2010.12.016
关键词
Microfluidics; Short tandem repeat analysis; Deoxyribonucleic acid identification; Forensics
Microfluidic technology has been utilized in the development of a modular system for DNA identification through SIR (short tandem repeat) analysis, reducing the total analysis time from the similar to 6 h required with conventional approaches to less than 3 h. Results demonstrate the utilization of microfluidic devices for the purification, amplification, separation and detection of 9 loci associated with a commercially-available miniSTR amplification kit commonly used in the forensic community. First, DNA from buccal swabs purified in a microdevice was proven amplifiable for the 9 miniSTR loci via infrared (IR)-mediated PCR (polymerase chain reaction) on a microdevice. Microchip electrophoresis (ME) was then demonstrated as an effective method for the separation and detection of the chip-purified and chip-amplified DNA with results equivalent to those obtained using conventional separation methods on an ABI 310 Genetic Analyzer. The 3-chip system presented here demonstrates development of a modular, microfluidic system for STR analysis, allowing for user-discretion as to how to proceed after each process during the analysis of forensic casework samples. (C) 2010 Elsevier B.V. All rights reserved.
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