期刊
ANALYTICA CHIMICA ACTA
卷 687, 期 2, 页码 184-192出版社
ELSEVIER
DOI: 10.1016/j.aca.2010.12.021
关键词
Molecularly imprinted polymers; Surface imprinting; Quartz crystal microbalance; Biosensor; Latex allergy
资金
- National Research Council of Thailand [PHA 51007]
- NANOTEC Center of Excellence at Prince of Songkla University, Thailand
- dean's office at the University of Vienna, Austria
Molecularly imprinted polymers (MIPs) for screening to detect rubber latex allergens (Hey b1) in natural rubber based products were designed as artificial recognition polymeric materials coated onto a quartz crystal microbalance (QCM). The polymers were prepared using a stamp imprinting procedure after mixing optimum amounts of methacrylic acid-vinylpyrrolidone-dihydroxyethylene bisacrylamide and Hey b1 latex allergen proteins, obtained from rubber gloves. QCM measurements showed that the resulting polymer layers after removal of the proteins used in their preparation could incorporate structures and features down to nanometer scale of protein templates into the imprinted polymer much better than a non-specific control polymer under controlled sensor conditions and an optimized polymerization process. This selective polymer but not the non-selective polymer clearly distinguished between the latex allergen Hey bl and proteins such as lysozyme, ovalbumin and bovine serum albumin, with a selectivity factor of from 2 to 4, and the response of the rubber elongation factors by an astonishing factor of 12. The imprinted cavities recognized specific binding sites and could distinguish among related hevein latex allergenic proteins isolated from fresh natural rubber latex; Hey b1, Hey b2, and Hey b3 with a selectivity factor of from 4 to 6. The different QCM measurements obtained presumably reflected slightly different conformations and affinities to the MIP binding sites. The sensor layers selectively adsorbed Hey b1 within minutes in amounts ranging from 10 to 1500 mu g L-1 and with a detection limit of 1 mu g L-1. This work has demonstrated that this new sensor provides a fast and reliable response to natural rubber latex protein, even after being extracted from the matrix of rubber gloves. (C) 2010 Elsevier B.V. All rights reserved.
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