期刊
ANALYTICA CHIMICA ACTA
卷 684, 期 1-2, 页码 121-125出版社
ELSEVIER
DOI: 10.1016/j.aca.2010.10.038
关键词
Aptamer; Adenosine; Electrochemiluminescent biosensor; Ru(bpy)(3)(2+)-doped silica nanoparticles
资金
- National Basic Research Program of China [2010CB732403]
- National Nature Sciences Funding of China [10802062, 20735002, 40940026, 20905013]
- Special Foundation for Young Scientists of Fujian Province, China [2008F3057]
A highly sensitive and selective electrochemiluminescent (ECL) biosensor for the determination of adenosine was developed. Single DNA (capture DNA) was immobilized on the gold electrode through Au-thiol interaction at first. Another DNA modified with tris(2,2'-bipyridyl) ruthenium(II)-doped silica nanoparticles (Ru-SNPs) that contained adenosine aptamer was then modified on the electrode surface through hybridizing with the capture DNA. In the presence of adenosine, adenosine-aptamer complex is produced rather than aptamer-DNA duplex, resulting with the dissociation of Ru-SNPs-labeled aptamer from the electrode surface and the decrease in the ECL intensity. The decrease of ECL intensity has a direct relationship with the logarithm of adenosine concentration in the range of 1.0 x 10(-10) to 5.0 x 10(-6) mol L-1. The detection limit of the proposed method is 3.0 x 10(-11) mol L-1. The existence of guanosine, cytidine and uridine has little interference with adenosine detection, demonstrating that the developed biosensor owns a high selectivity to adenosine. In addition, the developed biosensor also demonstrates very good reusability, as after being reused for 30 times, its ECL signal still keeps 91% of its original state. (C) 2010 Elsevier B.V. All rights reserved.
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