期刊
ANALYTICA CHIMICA ACTA
卷 697, 期 1-2, 页码 27-31出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.aca.2011.04.035
关键词
Click chemistry; Diazonium salts; Direct electron transfer; Horseradish peroxidase; Biosensor
资金
- National Natural Science Foundation of China [20875031]
- Shanghai Rising-Star Program [09QH1400800]
- New Century Excellent Talents in University [NCET-09-0357]
In this paper, a simple two-step approach for redox protein immobilization was introduced. Firstly, alkynyl-terminated film was formed on electrode surface by electrochemical reduction of 4-ethylnylphenyl (4-EP) diazonium compound. Then, horseradish peroxidase (HRP) modified with azido group was covalently immobilized onto the electrografted film via click reaction. Reflection absorption infrared (RAIR) spectroscopy and electrochemical methods were used to characterize the modification process. The results indicate that HRP retains its native structure and shows fast direct electron transfer. Moreover, the immobilized HRP shows excellent electrocatalytic reduction activity toward H(2)O(2) with a linear range of 5.0 x 10(-6) to 9.3 x 10(-4) mol L(-1). (C) 2011 Elsevier B.V. All rights reserved.
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