期刊
BIOSENSORS & BIOELECTRONICS
卷 23, 期 1, 页码 135-139出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2007.03.014
关键词
ZnO; nanoclusters; glucose biosensor; cross-linking
ZnO:Co nanoclusters were synthesized by nanocluster-bearn deposition with averaged particle size of 5 nm and porous structure, which were for the first time adopted to construct a novel amperometric glucose biosensor. Glucose oxidase was immobilized into the ZnO:Co nanocluster-assembled thin film through Nafion-assisted cross-linking technique. Due to the high specific active sites and high electrocatalytic activity of the ZnO:Co nanoclusters, the constructed glucose biosensor showed a high sensitivity of 13.3 mu A/mA cm(2). The low detection limit was estimated to be 20 mu M (S/N = 3) and the apparent Michaelis-Menten constant was found to be 21 mM, indicating the high affinity of the enzyme on ZnO:Co nanoclusters to glucose. The results show that the ZnO:Co nanocluster-assembled thin films with nanoporous structure and nanocrystallites have potential applications as platforms to immobilize enzyme in biosensors. (C) 2007 Elsevier B.V. All rights reserved.
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