Molecularly imprinted polymer-based chemiluminescence array sensor for the detection of proline

A new molecularly imprinted polymer (MIP)-chemiluminescence (CL) method has been developed for detection of praline. The molecularly imprinted polymer microspheres were synthesized using precipitation polymerization with hydroxyproline, a structural analogue, as the template. Polymer microspheres were immobilized in microtiter plates (96 wells) which selectively adsorbed the analyte (dansyl-proline). After washing, the bound fraction was quantified based on peroxyoxalate chemiluminescence (PO-CL) reaction enhanced by imidazole. The cavity of MIP synthesized with hydroxyproline as template is smaller, which can avoid non-specific adsorption and lead to enhancement of specificity, response speed and sensitivity when recognizing dansyl-proline. Under the optimum conditions, the relative CL intensity has a linear relationship with the concentration of proline in the range of 1 x 10(-6) mol L-1 to 4 x 10(-5) mol L-1, with a limit of detection 3 x 10(-7) mol L-1 (3 sigma). The relative standard deviation (R.S.D.) for praline (1 x 10(-6) mol L-1, n = 7) was 3.7%. The MIP-CL method can become a useful analytical technology for determination of praline in real sample. Crown Copyright (C) 2010 Published by Elsevier B.V. All rights reserved.