期刊
ANALYTICA CHIMICA ACTA
卷 666, 期 1-2, 页码 76-82出版社
ELSEVIER
DOI: 10.1016/j.aca.2010.03.051
关键词
Enzyme linked immunosorbent assay; Simultaneous determination; Carbaryl; Metolcarb; Matrix effects; Agricultural products
资金
- Ministry of Science and Technology of the People's Republic of China [2009ZX09502-027, 2009BADB9B03]
A direct competitive enzyme linked immunosorbent assay in multi-enzyme tracers format for the simultaneous analysis of carbaryl and metolcarb in agricultural products is described in this study. The concentrations of coating antibodies and enzyme tracer were studied. Under the optimum conditions, the limits of detection of carbaryl and metolcarb were 0.15 mu g L-1 and 1.2 mu g L-1, respectively. Determination of carbaryl and metolcarb in fruit juices and vegetables was accomplished by simple, rapid and efficient extraction methods. Recoveries of spiked samples were great than 70%. Validation of the immunosorbent assay was conducted by comparison of results from high performance liquid chromatography (HPLC). The correlations between the data obtained using multi-enzyme tracers enzyme linked immunosorbent assay and high performance liquid chromatography were good. Results indicated that the new strategy for developing immunoassay for simultaneous quantitative determination of carbaryl and metolcarb residues was suitable in this study. (C) 2010 Published by Elsevier B.V.
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