期刊
JOURNAL OF BIOLOGICAL CHEMISTRY
卷 282, 期 35, 页码 25884-25892出版社
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
DOI: 10.1074/jbc.M702446200
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Gelsolin regulates the dynamic assembly and disassembly of the actin-based cytoskeleton in non-muscle cells and clears the circulation of filaments released following cell death. Gelsolin is a six-domain ( G1 -G6) protein activated by calcium via a multi-step process that involves unfolding from a compact form to a more open form in which the three actin-binding sites ( on the G1, G2, and G4 subdomains) become exposed. To follow the global structural changes that accompany calcium activation of gelsolin, small-angle x-ray scattering (SAXS) data were collected for full-length human plasma gelsolin at nanomolar to millimolar concentrations of free Ca-2(+). Analysis of these data showed that, upon increasing free Ca2 (+) levels, the radius of gyration (Rg) increased nearly 12 A, from 31.1 +/- 0.3 to 43 +/- 2A, and the maximum linear dimension ( Dmax) of the gelsolin molecule increased 55 A, from 100 to 155 A. Structural reconstruction of gelsolin from these data provided a striking visual tracking of the gradual Ca2(+)- induced opening of the gelsolin molecule and highlighted the critical role played by the flexible linkers between homologous domains. The tightly packed architecture of calcium-free gelsolin, seen from both SAXS and x-ray crystallographic models, is already partially opened up in as low as 0.5 nm Ca-2(+) . Our data confirm that, although the molecule springs open from 0 to 1 mu M free Ca2 (+), even higher calcium concentrations help to stabilize a more open structure, with increases in Rg and Dmax of similar to 2 and similar to 15 A, respectively. At these higher calcium levels, the SAXS-based models provide a molecular shape that is compatible with that of the crystal structures solved for Ca2(+)/gelsolin C-terminal and N-terminal halves +/- monomeric G-actin. Placement of these crystal structures within the boundaries of the SAXS-based model suggests a movement of the G1/G2 subunits that would be required upon binding to actin.
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