期刊
BIOLOGY OF REPRODUCTION
卷 77, 期 3, 页码 551-559出版社
SOC STUDY REPRODUCTION
DOI: 10.1095/biolreprod.107.061358
关键词
acrosome reaction; calcium; sperm capacitation; sperm motility and transport
资金
- NEI NIH HHS [R01 EY013434, 1R01EY013434] Funding Source: Medline
- NICHD NIH HHS [1R03HD045290, 1R01HD047578] Funding Source: Medline
Many Ca2+ channel proteins have been detected in mammalian sperm, but only the four CATSPER channels have been clearly shown to be required for male fertility. Ca (2+) entry through the principal piece-localized CATSPER channels has been implicated in the activation of hyperactivated motility. In the present study, we show that the Ca (2+) entry also triggers a tail-to-head Ca 2+ propagation in the mouse sperm. When activated with 8-Br-cAMP, 8-Br-cGMP, or alkaline depolarization, a CATSPER-dependent increase in intracellular Ca 2+ concentration starts in the principal piece, propagates through the midpiece, and reaches the head in a few seconds. The Ca2+ propagation through the midpiece leads to a Ca (2+)-dependent increase in NADH fluorescence. In addition, CatSper1-mutant sperm have lower intracellular ATP levels than wild-type sperm. Thus, a Ca2+ influx in the principal piece through CATSPER channels can not only initiate hyperactivated motility, but can also trigger a tail-to-head Ca 2+ propagation that leads to an increase in [NADHI and may regulate ATP homeostasis.
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