期刊
ANALYTICA CHIMICA ACTA
卷 677, 期 1, 页码 24-28出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.aca.2010.01.028
关键词
Thiocyanate; Protein adducts; Biomarker; Gas chromatography-mass spectrometry; Extraction; Bioanalysis; Derivatization
资金
- US Army Medical Research Institute of Chemical Defense [DAAD19-02-D-0001]
- NIH/USAMRICD [Y1-A1-6176-03/A120-B.P2008-01]
- National Institutes of Health
- Department of Defense
Exposure to cyanide can occur in a variety of ways, including exposure to smoke from cigarettes or fires, accidental exposure during industrial processes, and exposure from the use of cyanide as a poison or chemical warfare agent. Confirmation of cyanide exposure is difficult because, in vivo, cyanide quickly breaks down by a number of pathways, including the formation of both free and protein-bound thiocyanate. A simple method was developed to confirm cyanide exposure by extraction of protein-bound thiocyanate moieties from cyanide-exposed plasma proteins. Thiocyanate was successfully extracted and subsequently derivatized with pentafluorobenzyl bromide for GC-MS analysis. Thiocyanate levels as low as 2.5 ng mL(-1) and cyanide exposure levels as low as 175 mu g kg(-1) were detected. Samples analyzed from smokers and non-smokers using this method showed significantly different levels of protein-bound thiocyanate (p<0.01). These results demonstrate the potential of this method to positively confirm chronic cyanide exposure through the analysis of protein-bound cyanide in human plasma. (C) 2010 Elsevier B.V. All rights reserved.
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